Addgene Viral Vectors Survey

We are conducting a survey to find out more about your AAV usage. Your feedback will help us improve our services and expand access to useful research materials. This survey should take less than five minutes. If you have any questions about the survey, please email us at klaus@addgene.org.

Question Title

* 1. Which type(s) of AAVs do you use for your research? Select all that apply.

Gene overexpression

Gene regulation (e.g., shRNA)

Gene editing / CRISPR

Recombinases (e.g., Cre)

Opto-/ chemogenetics

Genetic biosensors

Controls

Fluorescent proteins

Other (please specify)

Question Title

* 2. What AAV serotype(s) do you use for your research? Select all that apply.

AAV1

AAV2

AAV5

AAV8

AAV9

AAVrh10

PHP.eB

PHP.s

AAV-DJ

MaCPNS1 and/or MaCPNS2

CAP-B10 and/or CAP-B22

Other (please specify)

Question Title

* 3. What AAV titer do you most commonly use in a study? Please consider both experimental replicates and controls when answering.

< 1E+12 GC/mL

1E+12 GC/mL

5E+12 GC/mL

1E+13 GC/mL

>1E+13 GC/mL

Question Title

* 4. For the titer you chose above, what is the total volume of AAV preparation that you most commonly use in a study? Please consider both experimental replicates and controls when answering.

<100 µL

100 µL - 500 µL

500 µL - 1 mL

1 mL - 2.5 mL

2.5 mL - 5 mL

>5 mL

Question Title

* 5. How much would you expect to pay for an AAV preparation at the titer and volume you chose in the previous questions?

$300

$15,000

Clear

i We adjusted the number you entered based on the slider’s scale.

Question Title

* 6. When ordering an AAV preparation, are you willing to pay an extra fee for any of the following quality control add-ons? Select all that apply.

Purity/sterility assays (up to $170 per AAV preparation)

Endotoxin assay (up to $100 per AAV preparation)

Functional testing (e.g., transducibility, transgene expression) (up to $30 per AAV preparation )

Sequencing on the final viral vector preparation (up to $50 per AAV preparation)

Other (please specify)

None of the above

Question Title

* 7. Based on the information you have, please estimate what percentage of your laboratory’s AAV preparations come from custom viral packaging services.

100%

Clear

i We adjusted the number you entered based on the slider’s scale.

Question Title

* 8. In addition to custom viral packaging, what other services do you use? Select all that apply.

GMP-grade manufacturing

Custom cloning of transfer plasmid

In-vivo / Animal testing

Large-scale preparations

DNA preparation of transfer plasmid

Additional Quality Control Testing

Other (please specify)

None of the above

Question Title

* 9. When ordering custom viral packaging services, what starting materials do you provide to the vendor, if any? Select all that apply.

Transfer plasmid

Packaging plasmid (encoding a specific capsid)

Cell line

Other (please specify)

None of the above

Question Title

* 10. When using AAV preparations in your experiments, are there any unmet needs you want to share with us?

Question Title

* 11. Thank you for filling out our survey! Please share your information and enter your email address if you would like to be contacted by Addgene about your answers.