Join Philp Hoang for a personalized technical seminar to learn more about:
Rapid analysis of Glycosylated Antibodies by FcR Affinity Chromatography
Glycosylated proteins used as biopharmaceuticals, including monoclonal antibodies (mAbs), are among the most promising candidates in the drug development pipeline. Glycosylation is a critical factor in drug product solubility, kinetics, stability, efficacy, and immunogenicity. Understanding the inherent heterogeneity of N-glycan moieties of the Fc region of mAbs is an important part of cell line development and ultimately in the manufacture and quality control of these active pharmaceutical ingredients. Analytical methods utilize a suite of chromatographic modes using high performance liquid chromatography (HPLC) to analyze glycosylation of both intact and digested protein molecules.
The TSKgel® FcR-IIIA-NPR column is a high performance affinity chromatography column for the analysis of IgG glycoforms. The stationary phase utilizes a recombinant FcR-IIIA protein bound to a nonporous polymethacrylate polymer. The retention mechanism is based on the interaction between the FcR ligand and the sugar moieties attached to the ASN amino acid in the conserved region of the monoclonal antibody.
Register for this free personalized technical seminar to discover how this column technology that combines the specificity of the FcγIIIa receptor (FcγRIIIa) with the easy handling of an HPLC method can meet your lab’s need for the characterization of glycoforms.
This is a great opportunity to discuss the challenges in your analysis of glycosylated antibodies – it’s intended to be an interactive forum!
Philip is a Technical Marketing Specialist at Tosoh Bioscience whose background from different biotechnology companies range from quality control of pharmaceutical products to analytical development and process optimization of mAbs and ADCs.
He began at Tosoh Bioscience as an Account Manager for analytical products and now uses his in depth experience to support flow solutions related to Analytical Columns and Light Scattering Instruments with a focus on applications related to the characterization of proteins and large molecules.
Once registered, you will receive a phone call to set up your seminar.
